Required Practical: Chromatography
Required Practical: Chromatography
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You can carry out chromatography to separate and identify different components in a mixture such as INK or FOOD colorING.
Let's look at a chromatogram on an ink and 3 different dyes:
You can see that the ink has 3 DYES, two of which are known and one that is unknown.
You can tell by matching the heights of the components and assuming the ones at the SAME HEIGHTS have the same Rf values, so are the SAME SUBSTANCES.
Therefore the ink contains DYE 1 and DYE 2 but does NOT contain DYE 3, as it is at a completely different height to the others.
You can carry out chromatography to separate and identify different components in a mixture such as INK or FOOD colorING.
Let's look at a chromatogram on an ink and 3 different dyes:
You can see that the ink has 3 DYES, two of which are known and one that is unknown.
You can tell by matching the heights of the components and assuming the ones at the SAME HEIGHTS have the same Rf values, so are the SAME SUBSTANCES.
Therefore the ink contains DYE 1 and DYE 2 but does NOT contain DYE 3, as it is at a completely different height to the others.
